Augsburg 2006 – scientific programme

Parts | Days | Selection | Search | Downloads | Help

K: Kurzzeitphysik

K 1: Neue Verfahren I

K 1.4: Talk

Monday, March 27, 2006, 16:15–16:30, 1003

Imaging Neuronal Activity with Femtosecond Lasers — •Bruno E. Schmidt¹, Tobias Gleitsman¹, Tilman Franke², Thorsten M. Bernhardt^1,3, and Ludger Wöste¹ — ¹Institut für Experimentalphysik, Freie Universität Berlin, Arnimallee 14, D-14195 Berlin — ²Institut für Neurobiologie, Freie Universität Berlin, Königin-Luise-Straße 28/30, D-14195 Berlin — ³Abteilung Oberflächenchemie und Katalyse, Universität Ulm, D-89069 Ulm

The technique of two photon fluorescence excitation in combination with a laser scanning microscope (LSM) is applied to observe neuronal activity. This improved setup was used for observation of spatiotemporal dynamics of odor responses in living honeybee olfactory neurons via the calcium imaging method. Enhancement of fluorescence in combination with protection of biological tissue was achieved by optimizing laser pulse parameters and introducing a pulse-picker. We measured near transform limited 50 fs-pulses with high intensity in the focal plane of the microscope. Furthermore we report on results of pulse shaping in order to increase image quality of the microscope.