Dresden 2006 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe
AKB: Biologische Physik
AKB 11: Cell Adhesion I
AKB 11.4: Vortrag
Dienstag, 28. März 2006, 12:45–13:00, ZEU 260
Experiment and Modelling of Pattern Development during Fibronectin Nanofibril Formation — •Tilo Pompe1,2, Jörn Starruß3, Manfred Bobeth4, Wolfgang Pompe4,2, and Carsten Werner1,2 — 1Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany — 2Max Bergmann Center of Biomaterials Dresden — 3Institut für Lebensmittel- und Bioverfahrenstechnik, Technische Universität Dresden, 01062 Dresden, Germany — 4Institut für Materialwissenschaft, Technische Universität Dresden, 01062 Dresden, Germany
Adherent endothelial cells reorganize fibronectin molecules in the extracellular space into an ordered fibrillar network with characteristic patterns on the microscale as well as on the nanoscale. Cell culture experiments on polymer substrates with a gradated physicochemistry yield a dependence of fibronectin fibril pattern on the modulated anchorage strength of fibronectin to the substrates. The distinct spacing of fibronectin fibrils observed on the nanometer scale by scanning force microscopy can be correlated to the force sensitivity of the adhesion apparatus of the cell and the inner structure of the actin stress fibres [1].
To support this idea a stochastic model has been developed to explain the nanoscale observation of paired nanofibrils as a result of diffusion-controlled aggregation and myosin-driven transport of fibronectin-integrin complexes. The evolving patterns of fibronectin clusters and fibrils can be summarized in a morphological diagram as a function of fibronectin-substrate and fibronectin-fibronectin interaction energies.
[1] Pompe T, Renner L, Werner C (2005) Biophys. J. 88:527-534.