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AKB: Biologische Physik

AKB 12: Soft-Matter Nanofluidic Devices

AKB 12.5: Vortrag

Dienstag, 28. März 2006, 15:15–15:30, ZEU 255

High-Throughput Microfluidic Delivery of Suspended Cells for Marker-Free Deformability Measurement — •Bryan Lincoln — Institute for Soft Matter Physics, University of Leipzig, Linne’strasse 5, 04317 Leipzig

Microfluidic channels typically have the advantage of being laminar flow systems, meaning that are both reversible and linear. This enables their use as an efficient method of cellular transport. By incorporating a dual-beam laser trap, or optical stretcher, into a capillary-based microfluidic chamber, suspended cells can be serially delivered to the trap location where they undergo a step-stress deformation experiment. This deformability is a sensitive, quantitative measure of a cell’s global cytoskeletal organization and can be used to track cytoskeletal alterations during both physiological and pathological changes. Applications include the study of the progression of cancer, the differentiation of stem cells, the effects of cell culture conditions and cell cycle, and the evolution of primary cells in culture. This is a marker-free technique with potential for efficient sorting with minimal damage.