Dresden 2006 – scientific programme

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AKB: Biologische Physik

AKB 15: Biopolymers II

AKB 15.4: Talk

Tuesday, March 28, 2006, 17:15–17:30, ZEU 260

Vibrational imaging of single type I collagen fibrils by multiplex CARS microscopy — •Adam Muschielok, Marina Kovaleva, Alexander Kovalev, and Andreas Volkmer — 3rd Institute of Physics, University of Stuttgart, Pfaffenwaldring 57, 70550 Stuttgart

Collagen, one of the most abundant proteins in the extracellular matrix of animals, is important for the stability of tissues. Furthermore, because of its ability to self-assemble into fibrils, it is used as a model system for the formation of biopolymer structures. We have employed coherent anti-Stokes Raman scattering (CARS) microscopy in order to study the vibrational properties of individual in-vitro grown type I collagen fibrils. CARS microscopy allows the non-invasive imaging of collagen fibrils with high sensitivity, with three-dimensional sectioning capability, and with high spatial resolution. Beyond imaging, a multiplex CARS scheme was employed for the fast acquisition of CARS spectra in the region of the C-H stretching vibration. A detailed spectral analysis will be presented, which yields a representation of characteristic Raman line shape parameters in space, directly revealing the chemical and physical properties of a single collagen fibril in solution.