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AKB: Biologische Physik
AKB 20: Nano-Biomaterials and Devices
AKB 20.9: Vortrag
Donnerstag, 30. März 2006, 12:15–12:30, ZEU 255
A Novel Method to Fabricate Continuous Surface Tethered Membranes — •Christian Daniel1, Luisa Andruzzi2, Kirstin Seidel2, Joachim Rädler2, Erich Sackmann1, and Bert Nickel2 — 1Techn. Univ. Munich, Physics Dep. E22, James-Franck-Str. 1, 85748 Garching — 2L. M. Universität, Dep. f. Physik LS J. Rädler, Geschw.-Scholl-Pl. 1, 80539 München
Functionalization of solid surfaces (semiconductors, metal coated supports, electrooptical devices) with polymer supported lipid membranes provides a promising strategy to generate bio-analogue interfaces between inorganic and biological materials for scientific and practical applications. This work reports a new method for the generation of lipid bilayers separated from the surface by an ultra-thin soft polymer cushion mimicking the role of e.g. actin cortices of cell envelopes. The stratified films are fabricated by covalent anchorage of lipids on the solid surface and passivation of the uncovered surface with hydrophilic polymer films of different thickness. Spreading of vesicles on these activated surfaces results in the formation of supported membranes which are continuous over large areas and are characterized by very high long range mobility of constituents. Structural studies of the supported membranes by synchrotron x-ray reflectivity allow the determination of the thicknesses of the different interfacial films with sub-nanometer resolution. The major advantage of the tethered membranes is the reproducibility of the bilayer deposition procedure, which is expected to greatly facilitate the investigation of specific lipid-protein interaction mechanisms and their role for the self assembly and function of biological membranes.