Dresden 2006 – scientific programme

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AKB: Biologische Physik

AKB 27: Cell Mechanics II

AKB 27.6: Talk

Friday, March 31, 2006, 12:45–13:00, ZEU 260

Viscoelasticity and motility of murine fibroblasts transfected with additional filamin and actin measured with AFM-based microrheology technique — •Karla Mueller, Claudia Brunner, Bernd Kohlstrunk, Jens Gerdelmann, and Josef A. Kaes — Institut für Experimentelle Physik I, Physik der weichen Materie, Universität Leipzig, Linnestr 5, 04103 Leipzig

Malignantly transformed SVT-2 fibroblasts exhibit enhanced motility of the lamellipodium and decreased viscoelastic strength compared to normal fibroblasts. These properties are the result of an altered cytoskeleton. SVT-2 serve as a model cell line for cancer cells with a high metastatic potential. We present our efforts to reduce malignant cell motility by the insertion of additional cytoskeletal components with the goal to find a way to stop cancer metastasis. We transfected SVT-2 with actin to increase the number of filaments and filamin to increase the crosslinker density and probed the cell lines for their viscoelastic behaviour using the AFM microrheology technique as well as for the speed of lamellipodial extension.