Dresden 2006 – scientific programme

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AKB: Biologische Physik

AKB 30: Poster Session I

AKB 30.13: Poster

Monday, March 27, 2006, 15:30–18:00, P1

Intracellular CARS spectral imaging. — •Alexander Kovalev, Patincharath Nandakumar, and Andreas Volkmer — 3rd Institute of Physics, University of Stuttgart, Pfaffenwaldring 57, 70550 Stuttgart

For cellular components that either do not fluoresce or cannot tolerate the toxicity associated with staining and the photo bleaching of fluorophores, their intrinsic chemical properties can be used as contrast mechanisms through coherent anti-Stokes Raman scattering (CARS) microscopy. We report on the noninvasive vibrational imaging and microspectroscopic study of individual intracellular compartments within live eucaryotic cells. The spectroscopic information recorded by means of multiplex CARS microscopy allows differentiating between cellular organelles. The internal state and changes in composition of the organelles and cytoplasm could be monitored within range of the CH-stretching vibrations between 2800 cm-1 and 3000 cm-1. Examples of spectra from intracellular compartments in yeast cells and in differentiated 3T3 L1 cells will be presented.