Dresden 2006 – scientific programme

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AKB: Biologische Physik

AKB 40: Poster Session II

AKB 40.31: Poster

Wednesday, March 29, 2006, 16:30–19:30, P3

Orientation of the Membrane-Active Peptide Gramicidin S within Model Membranes — •Stefan Surber — Universität Leipzig, Lehrstuhl für die Physik Weicher Materie, Fakultät für Physik und Geowissenschaften, Linnestr. 5, D-04103 Leipzig

S. Surber, J.A. Käs and C. Selle

The amphipathic peptide gramicidin S (GS) attacks the integrity of membranes but the detailed mode of action is still unclear. In order to unravel the orientation of GS within models for bacterial and mammalian membranes - which is considered crucial for fundamental interactions between GS and membrane lipids - we performed an attenuated total reflection (ATR) Fourier transformed infrared (FTIR) spectroscopic study. For this purpose, mixtures from GS and phospholipid from neutral and anionic phospholipids representing bacterial and mammalian membrane components were used. The effect of varied hydration on the peptide-lipid interactions was also investigated. For the first time, the orientation of GS within model membranes was directly measured utilizing the related amide I IR spectroscopic absorptions. First results on the orientation of GS in Langmuir-Blodgett transferred lipid monolayers are also presented.