Dresden 2006 – scientific programme
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AKB: Biologische Physik
AKB 40: Poster Session II
AKB 40.59: Poster
Wednesday, March 29, 2006, 16:30–19:30, P3
Fluorescence analysis of environmental stress response of single cells within a bacterial population — •Judith Leierseder1, Kirsten Jung2, and Joachim Rädler1 — 1Physik Department LMU — 2Biologie Department LMU
Gene regulatory networks play a crucial role for the survival of bacteria as they allow them adaptation to varying environmental conditions. In our study we investigate the dynamics of gene expression in bacteria monitored by quantitative and time resolved image processing of GFP-hybrid proteins. This allows the acquirement of time traces of distinct proteins produced in response to environmental stress, which are used as input data for mathematical models. The analysis of many traces in an automated procedure measures the distribution of gene expression in a bacterial population. It allows conclusions on the regulatory mechanism and resolves cell-to-cell variations within a population. As a model system we studied the gene encoding GFP under the control of the arabinose promoter in E.coli. The change in GFP content following the exposure to different concentrations of arabinose was measured.