Dresden 2006 – scientific programme
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AKB: Biologische Physik
AKB 40: Poster Session II
AKB 40.64: Poster
Wednesday, March 29, 2006, 16:30–19:30, P3
Surface-diffraction from proteins interacting with solid supported membranes — •Kirstin Seidel1, Christian Daniel 2, Bert Nickel1, and Joachim Rädler1 — 1Ludwig-Maximilians Universität, Department für Physik and Center for Nanosciences, München — 2Technische Universität München
We study the interaction of Annexin 2 with anionic lipid membranes using neutron and x-ray synchrotron scattering techniques. Annexin 2 interacts with negatively charged phospholipids in a calcium dependant manner. Furthermore annexin is able to bind two adjacent membranes. The solid supported membranes were achieved by spreading large unilamelar vesicles (100nm) of lipid mixtures of neutral (POPC) and negatively charged (POPS) phospholipids on silicon substrates. The protein was provided by B. Windschiegl and C.Steinem, Universität Regensburg. The system was characterized by fluorescence microscopy the same samples were then measured with x-ray reflectivity at Hasylab (D4). With these methods we gained information about the homogeneity, fluidity and thickness of the underlying membrane. In future experiments we will use neutron reflectometry at FRM2 (REFSANS) to visualize the protein layer. Here we want to benefit from the possibility of contrast variation due to the scattering length density difference between deuterium oxide and water.