Dresden 2006 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 40: Poster Session II
AKB 40.67: Poster
Mittwoch, 29. März 2006, 16:30–19:30, P3
Imaging of Electrical Dynamics in Cultured Brain Slices by Multi-Transistor-Array (MTA) Recording — •Armin Lambacher1, Michael Hutzler1, Martin Jenkner2, Björn Eversmann2, Roland Thewes2, and Peter Fromherz1 — 1Max Planck Institute for Biochemistry, Department of Membrane and Neurophysics — 2Infineon Technologies, Corporate Research, München, Germany
Direct electrical interfacing of semiconductor chips with neuronal tissue may lead to novel experimental approaches in brain research and also give rise to hybrid computational devices. Here we report on a time-resolved imaging of the electrical activity in organotypic brain slices from rat hippocampus by multi-transistor-array (MTA) recording on an area of 1 mm2 at a resolution of 7.8 µm and 0.5 ms. Brain slices were cultured on the inert titanium dioxide surface of silicon chips fabricated by an extended CMOS process. Upon stimulation in the CA3 region we observed fast propagating waves of negative field potentials which we assign to orthodromic and antidromic action potentials in the mossy fibers and slower transient field potentials of postsynaptic activity in CA3 and CA1 with negative sign in stratum radiatum and positive sign in stratum pyramidale. The transistor signals matched local micropipette recordings of electrical field potentials in amplitude and shape. Direct interfacing of an MTA chip provides a complete observation of neuronal signaling in an extended area of brain tissue. This technique is suitable to elucidate the functionality of planar neuronal systems at a high resolution.