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AKB: Biologische Physik

AKB 40: Poster Session II

AKB 40.69: Poster

Mittwoch, 29. März 2006, 16:30–19:30, P3

Diffusion along Microfluidic Channels — •Andreas Heeren¹, Cheng-Ping Luo¹, Guenter Roth², Alexander Ganser³, Roland Brock³, Karl-Heinz Wiesmueller², Wolfgang Henschel¹, and Dieter Kern¹ — ¹Institute of Applied Physics, University of Tuebingen, 72076 Tuebingen, Germany — ²EMC microcollections GmbH, 72070 Tuebingen, Germany — ³Institute for Cell Biology, University of Tuebingen, 72076 Tuebingen, Germany

Living cells respond simultaneously to a variety of different stimuli. To achieve a specific cellular response a well defined mixture of stimuli or agents is required. However, in order to identify optimum mixtures of even few different compounds with respect to their relative and absolute concentrations, large numbers of biological tests are needed. Diffusion represents a highly efficient means for the generation of substance mixtures, namely continuously varying concentration profiles, with a minimum of pipetting steps. Here we present an array of microfluidic channels for the generation of binary substance mixtures with only two pipetting steps. For this purpose a microfluidic structure with a height of 500 µm was fabricated using the negative tone resist SU8. In a test diffusion of fluorescein dissolved in water was observed by fluorescence microscopy. The diffusion constant was determined by analyzing the fluorescence micrographs. Furthermore a procedure to detect unwanted flow in the channels was developed.