Dresden 2006 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe
AKB: Biologische Physik
AKB 9: Chemical Bonds and Adsorption
AKB 9.3: Vortrag
Dienstag, 28. März 2006, 11:30–11:45, ZEU 260
Characteristics of Protein Adsorption at Solid-Liquid Interfaces — •Hubert Mantz1, Armin Nagel1, Anthony Quinn2, and Karin Jacobs1 — 1FR 7.2 Experimental Physics - Soft Matter, Saarland University, D-66123 Saarbrücken — 2Department of Chemical and Biomolecular Engineering, University of Melbourne, Australia
Adsorption of proteins occurs instantly whenever a protein solution (e.g. any body fluid) gets in touch with a surface (biomaterials or medical devices) and thereby is of crucial importance to all kinds of biomedical technologies. Adsorption is a very complex process and is not yet fully understood. The adsorbed amount and the structure of the adsorbed layer depend on many factors including properties of the surface, the surrounding medium and the protein itself.
In this study, the in situ adsorption kinetics of some selected saliva proteins (amylase and lysozyme) in liquid environment has been studied by ellipsometry. Through judicious choice and modification of the substrates, the influence of short- and long-range forces on the adsorption process could be separated. The ellipsometry measurements were compared with AFM images of the resulting topographies.
The experimentally observed adsorption kinetics does not follow any standard adsorption model. We compare our results with Monte-Carlo simulations (cf. contribution of Bellion et al.), assuming conformational changes of the proteins upon increasing surface coverage.