Dresden 2006 – scientific programme
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CPP: Chemische Physik und Polymerphysik
CPP 12: POSTER Colloids, Nanoparticles and Self-Organizing Systems
CPP 12.26: Poster
Tuesday, March 28, 2006, 17:00–19:00, P3
The x-ray small angle scattering setup at beamline 9 at the synchrotron source DELTA, University of Dortmund: Measurements on Lysozyme solutions — •Christof Krywka1, Christian Sternemann1, Nadeem Javid2, Roland Winter2, and Metin Tolan1 — 1Institute of Physics / DELTA, University of Dortmund, D-44221 Germany — 2Institute of Chemistry, University of Dortmund, D-44221 Germany
The physical and chemical properties of proteins are influenced by their aqueous microenvironment. Dynamics of folding and aggregation processes play an important role in conformational diseases (e.g. Alzheimer, Parkinson). It was shown that the latter processes are in fact influenced by the type and concentration of cosolvents. As a part of our immune system Lysozyme breaks carbohydrate chains, destroying the structural integrity of bacteria cell wall. Small-angle X-ray scattering (SAXS) measurements on aqueous solutions of Lysozyme were performed in a wide range of concentrations and in presence of different types of cosolvents, typical for conditions where unfolding sets in within cells. We determined the effects of cosolvents on the unfolded state structures and aggregated structures. From the measured structure factors information about the structure of the hydration shell and, for the higher concentrated samples, information about the intermolecular interaction potential can be obtained.