Dresden 2006 – scientific programme

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CPP: Chemische Physik und Polymerphysik

CPP 13: POSTER New Experimental Techniques

CPP 13.8: Poster

Tuesday, March 28, 2006, 17:00–19:00, P3

High NA UV digital in-line holography — •Martina Schürmann¹, Hans Jürgen Kreuzer², Michael Grunze¹, and Axel Rosenhahn¹ — ¹Angewandte Physikalische Chemie, Universität Heidelberg, 69120 Heidelberg — ²Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Nova Scotia B3H 3/5, Canada

Holography is a lensless imaging technique with intrinsic three-dimensional properties. The radial divergence of spherical waves enables the acquisition of a magnified image of the sample. Unlike conventional microscopy, the method does not require lenses. The achievable resolution thus only depends on the wavelength of the light and the detection angle. With short wavelengths tiny objects can therefore easily be resolved. For the first time, UV laser light and a high numerical aperture setup were used in order to holographically enlarge transparent and opaque particles and characterize their imaging properties. Furthermore, fibroblast cells were imaged in order to explore future applications in biology. As the presence of the reference wave introduces the third dimension, first experiments demonstrating the three dimensional nature of the method are also shown. All results are compared to images obtained by conventional light microscopy.