Dresden 2006 – scientific programme

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CPP: Chemische Physik und Polymerphysik

CPP 25: POSTER Electronic Structure and Spectroscopy

CPP 25.12: Poster

Thursday, March 30, 2006, 17:00–19:00, P2

Setup for flowcytometric based fluorescence measurements of red fluorescent dyes — •Babette Hinkeldey, Alexander Schmitt, and Gregor Jung — Biophysical Chemistry, Saarland University, Building B2.2, D-66123 Saarbrücken, Germany

In the last decades, Single Molecule Detection (SMD) has become an important scientific field in biophysical and biomedical applications. By coupling individual molecules with fluorescent dyes, observation of the movements of these individual molecules even in living cells is possible. Due to reduced autofluorescence in living cells, red dyes are favoured. Thus, the understanding of the photophysical properties of fluorescent dyes is one of the relevant domains. Especially the photostability is of great interest, as a precise prediction of the average number of excitation cycles before dye degradation is highly recommended for quantitative and comparable results. In addition, the correlation between chemical structure and physical properties could result in improved strategies of synthesis of application matched dyes. Therefore it is necessary to develop a method which fulfills the conditions to obtain quantitative results in order to being able to compare different fluorescent dyes. In our contribution we show the setup for flowcytometric based fluorescence measurements. Finally, perfomance data and first results with a red fluorescent dye are presented.