Dresden 2006 – scientific programme

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CPP: Chemische Physik und Polymerphysik

CPP 25: POSTER Electronic Structure and Spectroscopy

CPP 25.14: Poster

Thursday, March 30, 2006, 17:00–19:00, P2

Photobleaching on the Single Molecule and Ensemble Level — •Jörg Brabandt, Jörg Schuster, and Christian von Borczyskowski — Institute of Physics, TU Chemnitz, 09107 Chemnitz

We study the photobleaching of dye molecules on the single molecule and ensemble level. Ensemble photobleaching is characterized by a reversible and an irreversible part, the latter one being the well established photodestruction of the dye. The reversible part of the photobleaching is due to the existence of dark periods where the molecule reversibly switches between on- and off-states, so called photoblinking [1] [2]. The discrimination between the two mechanisms is a difficult task, since the decision wether a given dye molecule is in a long dark state or already irreversibly photochemically destructed requires extremely long observation times. We show, how both aspects of photobleaching can be inferred from single molecule observations. Furthermore, we present a detailed analysis of the reversible part of photobleaching by a number of ensemble experiments.

[1] Orrit et al., J. Phys. Chem. A 107 (2003), 6770

[2] Schuster et al., Appl. Phys. Lett. 87 (2005), 051915