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CPP: Chemische Physik und Polymerphysik

CPP 6: Electronic Structure and Spectroscopy I

CPP 6.5: Vortrag

Montag, 27. März 2006, 15:45–16:00, ZEU 114

The Relation of Photoblinking and Photobleaching for Single Dye Molecules — •Jörg Schuster, Jörg Brabandt, and Christian von Borczyskowski — Institute of Physics, TU Chemnitz, D-09107 Chemnitz

An inherent fingerprint of single molecule detection is the observation of photoblinking on different time scales. In recent papers [1] we could show, that for several dye molecules a blinking mechanism exists, which is characterized by power law statistics of on- and off-times. The so called "power law blinking" is connected to an increasing duration of off-times during the observation of a single emitter. Thus, the discrimination between long dark periods and irreversible photodestruction of the molecules becomes impossible. In ensemble experiments the presence of dark periods manifests as reversible photobleaching [2].

In our contribution we present, how both types of photobleaching can be analyzed by single molecule observations. The suggested analysis is further supported by computer simulations which include power law blinking and irreversible photobleaching. Data are also discussed within the framework of a recent theory on the intermittency of semiconductor nanocrystals by Marcus et al. [3] which describes also several aspects of dye molecule blinking.

[1] Schuster et al., APL 87 (2005), 051915 and herein

[2] Orrit et al., J. Phys. Chem. A 107 (2003), 6770

[3] Marcus et al., PRL 95 (2005), 107401