Dresden 2006 – wissenschaftliches Programm

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DY: Dynamik und Statistische Physik

DY 41: Dynamical Physics in Biological Systems

DY 41.2: Vortrag

Donnerstag, 30. März 2006, 10:15–10:30, SCH 251

A Bayesian Approach to the Evaluation of Dynamic Force Spectroscopy Experiments — •Sebastian Getfert, Peter Reimann, and Martin Raible — Condensed Matter Theory, Universität Bielefeld, Universitätsstraße 25, 33615 Bielefeld

The components of a biomolecular complex can be connected via suitable linkers to the tip of an atomic force microscope and a moving surface. When this surface is pulled apart at constant velocity the force f that acts on the bond increases (approximately) linearly in time until the chemical bond ruptures. This process is of stochastic nature. The distribution of the rupture forces and in particular the maximum depends in a characteristic way on the loading rate ḟ and on binding parameters like dissociation length and (force-free) dissociation rate [1]. Thus the evaluation of rupture force data from dynamic force spectroscopy experiments allows to draw conclusions about the energy landscape of the bond. We present a Bayesian approach to this evaluation in which experimental uncertainties can be included in a natural way. Further we discuss from a statistical point of view to which degree of accuracy the parameters of the distribution can be determined.

[1] E. Evans and K. Ritchie, Biophys. J. 72, 1541 (1997)