Frankfurt 2006 – wissenschaftliches Programm
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MO: Molekülphysik
MO 71: Dynamics and Spectroscopy at Interfaces
MO 71.7: Vortrag
Freitag, 17. März 2006, 12:10–12:25, H12
Extreme Adsorption Capability of Nanodiamond and Detectability of Lysozyme Concentration by Fluorescence Method — •Victor Wei-Keh Wu1,2,3 and Kowa Chen1 — 1Inst. of Atomic and Molecular Sci., Academia Sinica, P.O.Box 23-166, 10617 Taipei Taiwan — 2Dept. of Chem. Eng., National Kaohsiung Univ. of Appl. Sci., 80782 Kaohsiung, Taiwan — 3Victor Basic Res. Lab. e. V., Gadderbaumer-Str. 22, 33602 Bi., Germany, http://www.che.kuas.edu.tw
UV-Absorbance has been applied usually, for resolution of Lysozyme (containing 4 tryptophanes) up to ca. 50-100 µM, via Sore band at 409 nm. The adsorption capability of Nanodiamond(ND) φ=100 nm - nearly all kinds of proteins in a sol. can be completely adsorbed1, and the detectability of extremely diluted lysozyme solution are demonstrated. Diff. conc. of lysozyme (M=14300 g/mole, Sigma Chemical) of chicken egg white betw. 1-500 nM were prepared in KPBS buffer sol. of 7 mM. Xenon Lamp (2500 W, L.P. Associates, Inc.) for 285 nm with 3 mm slit width was used. A quarz cuvette with 10x10 cross section was used. Sol. well stirred, fluorescence was collected perpendicularly with PMA-11 of Hamamatsu. The linearity of the detected fluorescence counts depend. upon the lysozyme conc. betw. 10-500 nM was well measurable. The ND used, was 5-20µg. The lysozyme adsorption capability by ND can be distinguishably followed down to 10 nM. This is 104 as powerful as by UV-Absorbance, and can be a new method for doping control2. ∗Wu is the correspondance author. Ref. 1. X.-L. Kong, et al., AC. 77, 259 (2005), 2. J. Wallrafen, GIT, 49, 644 (2005).