Düsseldorf 2007 – wissenschaftliches Programm

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MO: Fachverband Molekülphysik

MO 11: Biomolecules

MO 11.8: Vortrag

Montag, 19. März 2007, 15:45–16:00, 6B

PH Enhancement on the Adsorption Reaction of Nanodiamond/Nanosilica and Lysozyme Molecule — •Victor Wei-Keh Wu — Department of Chemical and Material Engineering, National Kaohsiung University of Applied Sciences, 80782 Kaohsiung, Taiwan — Group 510, Institute of Atomic and Molecular Sciences, Academia Sinica, P.O.Box 23-166, 10617 Taipei, Taiwan — Victor Basic Research Laboratory e. V. Gadderbaumer-Str. 22, 33602 Bielefeld, Germany, Email:victorbres3tw@yahoo.com.tw, http://www.che.kuas.edu.tw

Fluorescences from free lysozyme of 0-1000 nM in 7 mM PPBS at pH = 7, 9, 11 and 13 after adsorption reactions on the surfaces of nanodiamond/-silica (d≈100 nm, 5 µg/2.02 mL) with Xe lamp as light source monochromated at 285 nm of ca. 0.6 mW and PMA-11 of Hamamatsu as fluorescence spectrometer have been measured. Coverages as well as adsorption reaction constants (e.g. 1.6 x 10⁸/4.5 x 10⁷ [nM]⁻¹ for nanodiamond/-silica, respectively, at pH = 11) have been further obtained. Their quotients for both systems at pH = 7 - 13 is ca. 4. The max. appears at around 11 - 13, where lysozyme as No-dqneutralNo-dq (pI≈11.5) can be easierly adsorbed. The available surface areas of nanodiamond and -silica during adsorption for each lysozyme can be estimated as 10 and 2 nm², respectively. Comparing this ratio with the quotients of the measured adsorption reaction constants, roughness of particle surface may also be decisive besides charge on and modification of the surface, pH of the surrounding for higher adsorption capability. The adsorbed lysozyme on the surface of nanodiamond may better keep its helicity as well as its activity. Ref. V. W.-K. Wu, Chem. Lett. 35, 1380 (2006)