Düsseldorf 2007 – wissenschaftliches Programm

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MO: Fachverband Molekülphysik

MO 21: Femtosecond Spectroscopy II

MO 21.4: Vortrag

Dienstag, 20. März 2007, 11:30–11:45, 6D

Towards Femtosecond Stimulated Raman Microscopy (FSRM) — •Evelyn Plötz, Stefan Laimgruber, Stefan Berner, and Peter Gilch — Institut für BioMolekulare Optik, Department für Physik, Ludwig-Maximilians-Universität, Oettingenstr. 67, D-80538 München, Germany
Since the invention of microscopy diverse techniques to enhance the contrast of biological samples have been developed. In that respect, techniques relying on vibrational spectroscopy are very promising since the provide a contrast mechanism based on the chemical composition (chemical contrast). Because IR microscopy suffers from a low spatial resolution and (conventional) Raman microscopy from the low signal levels, non-linear Raman spectroscopy was applied for microscopy. Today the most important implementation is based on Coherent Anti-Stokes Raman Scattering (CARS) [1].

We here present a potential alternative to CARS microscopy, Femtosecond Stimulated Raman Microscopy (FSRM). In FSRM a femtosecond white light and narrow bandwidth laser pulse are focused onto the sample by a microscope objective. Stimulated Raman interaction superpose the Raman signature of the focal spot with the white light spectrum. By raster scanning the sample a FSRM micrograph can be recorded. Merits and pitfalls of FSRM in comparison with CARS microscopy will be discussed.

[1] J.-X. Cheng, X.S. Xie, J. Phys. Chem. B 108 (2004) 827