Düsseldorf 2007 – wissenschaftliches Programm

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MO: Fachverband Molekülphysik

MO 26: Poster: Biomolecules

MO 26.1: Poster

Dienstag, 20. März 2007, 16:30–18:30, Poster A

Saturation Threshold of Adsorption Reaction of Lysozyme on Surfaces of Nanodiamond/Nanosilica — •Victor Wei-Keh Wu — Department of Chemical and Material Engineering, National Kaohsiung University of Applied Sciences, 80782 Kaohsiung, Taiwan — Group 510, Institute of Atomic and Molecular Sciences, Academia Sinica, P.O.Box 23-166, 10617 Taipei, Taiwan — Victor Basic Research Laboratory e. V. Gadderbaumer-Str. 22, 33602 Bielefeld, Germany, Email:victorbres3tw@yahoo.com.tw, http://www.che.kuas.edu.tw

Fluorescences from free lysozyme of 0-1000 nM in 7 mM PPBS at pH = 11.0 after adsorption reactions on the surfaces of nanodiamond (100 nm, No.I-21, carboxylated; KDM, Kay Diamond) and nanosilica (100 nm, VP OX 10, degussa) with Xe lamp as light source monochromated (Tandem GM252, ABI Analytical) at 285 nm of ca. 0.6 mW and PMA-11 of Hamamatsu as fluorescence spectrometer have been measured. Each of 2 mL lysozyme solutions was treated with 50 µg suspension of nanodiamond/-silica in 20 µL PPBS. BET and Langmuir surface areas, 55 and 80 m²/g for nanodiamond, 15 and 20 m²/g for nanosilica, respectively, have been obtained by use of adsorptive dose rate, and static volumetric measurement technique, where BET surfaces are applicable. Saturation thresholds of adsorption of lysozyme have been measured at 190 and 175 nM; 110 and 101 mg lysozyme adsorbed for 1 g nanodiamond/-silica can be estimated, respectively. The adsorbed lysozyme on an unit surface of nanoparticle can finally be obtained as 2.0 and 1.8 [mg-lysozyme/m²-nanoparticle] for nanodiamond/-silica, respectively. Ref. V. W.-K. Wu, Chem. Lett. 35, 1380 (2006).