Regensburg 2007 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Micro- and Nanofluidics
BP 11.1: Vortrag
Dienstag, 27. März 2007, 16:00–16:15, H43
DNA Dielectrophoresis in Microfluidic Systems: Separation and Polarizability — •Henning Höfemann1, Jan Regtmeier1, Ralf Eichhorn2, Dario Anselmetti1, and Alexandra Ros1 — 1Bielefeld University, Biophysics & Applied Nanosciences, Universitätsstr. 25, 33615 Bielefeld, Germany — 2Bielefeld University, Condensed Matter Theory, Universitätsstr. 25, 33615 Bielefeld, Germany
Electrophoresis (EP) and dielectrophoresis (DEP) represent an important tool for the manipulation of DNA in microfluidic systems. Recently, we have demonstrated that the DEP and EP forces can be exploited in tailored microfluidic systems to separate long DNA strands and to quantitatively access their polarizabilities [1]. The microfluidic device includes periodically arranged rows of posts generating DEP traps for DNA upon application of an AC voltage. Further an additional DC voltage invokes migration of DNA through the microchannel. The subtle interaction of the DNA with the created energy landscape, tuned by the parameters of the DC offset and AC conditions (amplitude and frequency) allows a precise control of the length dependent migration and trapping of DNA. Here, we extend our studies and demonstrate the length dependent migration of covalently closed circle DNA in a size range between 7 and 23 kpb including the quantitative deduction of DNA polarizabilities. Our future work is dedicated to the exploitation of this novel migration mechanism to the separation of DNA varying in length and conformation. [1] J. Regtmeier, T. T. Duong, R. Eichhorn, D. Anselmetti, A. Ros; Dielectrophoretic Manipulation of DNA: Separation and Polarizability, 2006, submitted