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BP: Fachverband Biologische Physik
BP 14: Chemotaxis
BP 14.2: Talk
Tuesday, March 27, 2007, 14:45–15:00, H44
Cytoskeletal dynamics in response to complex temporal signals — •Carsten Beta1, Hellen Ishikawa2, Till Bretschneider2, Günther Gerisch2, and Eberhard Bodenschatz1 — 1MPI for Dynamics and Self-Organization, Göttingen, Germany — 2MPI of Biochemistry, Martinsried, Germany
Understanding of cytoskeletal dynamics has seen a rapid advance through the use of fluorescent fusion proteins. Further progress in this field relies on experimental techniques to stimulate single cells with high temporal resolution. We combine microfluidic techniques with the photo-chemical release of caged signaling agents to expose cells to well-defined stimuli with changing temporal patterns. We apply this approach to quantify intracellular translocation of various fluorescently labeled cytoskeletal proteins in chemotactic Dictyostelium cells responding to complex temporal stimuli with cAMP.