Regensburg 2007 – scientific programme

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BP: Fachverband Biologische Physik

BP 14: Chemotaxis

BP 14.3: Talk

Tuesday, March 27, 2007, 15:00–15:15, H44

Intracellular dynamics during directional sensing of chemotactic cells — •Gabriel Amselem, Eberhard Bodenschatz, and Carsten Beta — MPI for Dynamics and Self-Organization, Göttingen, Germany

We use an experimental approach based on the photo-chemical release of signaling molecules in microfluidic environments to expose chemotactic cells to well controlled chemoattractant stimuli. We apply this technique to study intracellular translocation of fluorescently labeled PH-domain proteins in the social ameba Dictyostelium discoideum. Single chemotactic Dictyostelium cells are exposed to localized, well defined gradients in the chemoattractant cAMP and their translocation response is quantified as a function of the external gradient.