Regensburg 2007 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 16: Poster Session I

BP 16.30: Poster

Dienstag, 27. März 2007, 17:00–19:30, Poster D

Ex-situ measurements of adsorbed protein layers — •Christof Weitenberg, Hubert Mantz, and Karin Jacobs — Saarland University, Experimental Physics, D-66041 Saarbrücken, Germany

Measurements of biological tissue are usually done in situ, i.e. in solution. We investigated the possibility of an ex-situ determination of the thickness of lysozyme protein layers adsorbed to silicon oxide surfaces by means of ellipsometry. The expected shape of the adsorption kinetics and isotherms for different ionic strengths could be reproduced. Yet the time scale for adsorption appears to be much larger than for comparable in-situ measurements. We discuss the possible explanations for this behavior as well as assets and drawbacks of the applied ex-situ procedure.