Regensburg 2007 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 16: Poster Session I
BP 16.43: Poster
Dienstag, 27. März 2007, 17:00–19:30, Poster D
Growth dependent alterations of the energy metabolism in neuronal cell cultures — •Jiraporn Luengviriya1, Thomas Mair1, Carina Helemeke2, Katharina Braun2, and Stefan C. Müller1 — 1Otto-von-Guericke-Universtität Magdeburg, Institut für Experimentelle Physik, Abteilung Biophysik, Universitätsplatz 2, 39106 Magdeburg, Germany — 2Otto-von-Guericke-Universtität Magdeburg, Institut für Biologie, Lehrstuhl für Zoologie/Entwicklungsbiologie, Brenneckestr.6, 39118 Magdeburg, Germany
Glycolysis is an essential pathway of the energy metabolism in astrocytes which supplies energy-rich intermediates for neurons, via the so called lactate shuttle, in order to maintain the energy fuel of neurons. We investigated the energy state of cell cultures from the hippocampus of new born rats as a function of their growth state by spatiotemporal recordings of NAD(P)H-fluorescence. We stimulated the cells by local application of different chemicals and found, that cyanid inhibition of respiration leads to a pronounced increase of NAD(P)H fluorescence. This response was growth dependent and increased until about 15 days. Thereafter it decreased again. With the neurotransmitter NMDA as stimulus, we found also an increase of NAD(P)H-fluorescence, but now with 2 optima. The first one between day 6 and 12 and a second one between days 17 to 24. At the optimum of cyanid stimulation there was no response of the cellular NAD(P)H to NMDA. We interpret these data such, that there is a switch between glycolytic and respiratory energy metabolism during growth of these cells.