Regensburg 2007 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 16: Poster Session I

BP 16.53: Poster

Dienstag, 27. März 2007, 17:00–19:30, Poster D

Investigtion of the first stepsat the CNTF mediated signal transduction by means of fluorescence correlation spectroscopy (FCS) in living cells — •Eva Wallhäußer¹, Felix Neugart¹, Andrea Zappe¹, Deborah Buk², Lutz Graeve², Carsten Tietz¹, and Jörg Wrachtrupp¹ — ¹3. Physikalisches Institut, Universität Stuttgart, Stuttgart — ²Institut für Chemische Biologie und Ernährungswissenschaften, Universität Hohenheim, Stuttgart

The investigation of signal transduction in living cells is an important step to understand what is happening in the body during the signal transduction and possibly influence several diseases in the case of a malfunction within the signalling cascade. Due to the tiny concentration of most of the signalling components a very sensitive method like FCS is necessary. In this work the first steps of signal transduction of the cilliary neutrophic factor (CNTF) by the CNTF-receptor complex were investigated. The complex is considered of three components, namely, CNTF-receptor itself, LIF-receptor and gp130. Measuring the diffusion constant of this different GFP-labeled receptors allows us to show if some parts of the receptor are pre-associated.