Regensburg 2007 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 19: Biosensors and Biofunctionalized Systems

BP 19.2: Vortrag

Mittwoch, 28. März 2007, 15:45–16:00, H43

Development of a Biosensor Device Comprising Functionalized Silicon-On-Insulator (SOI) Structures for the Specific Detection of Proteins — •Bernhard Wunderlich, Petra Neff, and Andreas Bausch — Lehrstuhl für Biophysik E22, TU München, 85747 Garching, Germany

Recently, a new Silicon-on-Insulator (SOI) based thin film resistor for chemical and biological sensor applications was introduced. Its response against pH changes and variations of the salt concentration was measured and compared to the theoretical predictions. It has been shown that this sensor is highly sensitive to variations of the surface potential evoked by the adsorption of small amounts of charged molecules.

We use this sensor device for the label-free detection of proteins. The passivation of the native silicon oxide surface by either physical adsorption of proteins or covalent binding of silane is presented. Different strategies for further functionalizations of the sensor surface with molecules for biomolecular recognition have been evaluated, including the deposition of lipid monolayers with incorporated metal chelate lipids and covalent immobilization of antibodies onto the sensor. Results of the specific detection of proteins by affinity reactions are discussed and compared to the results obtained from fluorescence and ellipsometry measurements.

As the device is based on standard semiconductor technologies, the SOI-based biosensor is well suited for parallelization needed in high throughput applications. We present a sensor device including several sensitive areas suitable for parallel and differential detection.