Regensburg 2007 – scientific programme

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BP: Fachverband Biologische Physik

BP 22: Molecular Machines

BP 22.12: Talk

Thursday, March 29, 2007, 13:00–13:15, H43

Regulation of a chimeric Eg5head/DmKHC tail motor protein — •Stefan Lakämper¹, Mikhail J. Korneev^2,3, Stefanie Reiter¹, Lukas C. Kapitein³, Erwin J.G. Peterman³, and Christoph F. Schmidt¹ — ¹III. Physikalisches Institut, Georg-August-Universität, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany — ²ASML, De Run 6501, 5504 DR Veldhoven, The Netherlands — ³Department of Physics and Astronomy and Laser Centre, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands

We have constructed a chimeric motor protein using the head and neck portion of the mitotic Eg5 kinesin and the tail protion of a kinesin 1 (DmKHC). This chimeric motor maintains characteristic features of the slow mitotic Eg5, but it is dimeric instead of tetrameric and the regulation by the opposing pair of heads is eliminated. This allowed us to study directly the inhibition of the dimer function by the small drug monastrol.