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Regensburg 2007 – scientific programme

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BP: Fachverband Biologische Physik

BP 26: Poster Session II

BP 26.13: Poster

Thursday, March 29, 2007, 17:00–19:30, Poster B

Single molecule studies of eukaryotic transcription using optical tweezers — •Adam Muschielok1, Joanna Andrecka1, Florian Brückner2,1, Patrick Cramer2,1, and Jens Michaelis11Departement Chemie und Biochemie, Ludwig-Maximilians-Universität München, Deutschland — 2Gene Center Munich, Ludwig-Maximilians-Universität München, Deutschland

Our goal is to study the molecular mechanisms of the eukaryotic transcription process. Therefore we monitor the activity of single RNA Polymerase II (RNAP) molecules during RNA elongation using optical tweezers. We are interested in the elongation process and in the effects of transcription cofactors on RNAP activity.

We present preliminary data together with simulations to discuss the data analysis.

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