Regensburg 2007 – scientific programme

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BP: Fachverband Biologische Physik

BP 26: Poster Session II

BP 26.1: Poster

Thursday, March 29, 2007, 17:00–19:30, Poster B

Photokinetics and photostability of fluorescent dyes — •Babette Hinkeldey, Gregor Jung, and Alexander Schmitt — Biophysical Chemistry, Saarland University, Building B2.2, D-66123 Saarbrücken, Germany

In biophysical applications, Fluorescence Correlation Spectroscopy (FCS) has become a well established method to investigate the photokinetics of fluorescent dyes. In combination with fluorescence lifetime measurements calculation of singulet and triplet state parameters is possible.

Yet a quantitative description of the photostability cannot easily be achieved by applying the FCS technique.Therefore a flow cytometric setup is used, where by means of a microcapillary and an electric field the dye molecules are forced into a specific direction. This leads to the possibility to observe the same molecule twice.

We expect to obtain a precise prediction of the average number of excitation cycles before photodegradation in dependence of the excitation intensity. Thus, combination of both techniques allows a more detailed understanding of fluorescence properties.