Regensburg 2007 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 26: Poster Session II

BP 26.25: Poster

Donnerstag, 29. März 2007, 17:00–19:30, Poster B

Methods for attaching individual metallic Nanoparticles on AFM Tips — •Olaf Schulz, Volker Walhorn, Christoph Pelargus, Dario Anselmetti, and Robert Ros — Experimental Biophysics, Physics Department, Bielefeld University, Germany

The combination of atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRFM) has proven a valuable tool for analyzing the interaction between single fluorophores and metallic nanoobjects [1]. Since energy transfer effects like fluorescence quenching play an increasing role in the investigation of inter- or intramolecular dynamics it is essential to understand the influence of different quenching agent properties (material, size or geometry) on these effects. Therefore it is crucial to attach single well defined particles to a cantilever tip.

We will discuss different methods of attaching single metallic nanoparticles to the very end of an AFM tip and the possibility to use them for distance controlled fluorescence intensity modulation with our combined AFM-TIRFM Setup.

[1] R. Eckel, V. Walhorn, Ch. Pelargus, J. Martini, J. Enderlein, Th. Nann, D. Anselmetti, and R. Ros; Small (in press).