Regensburg 2007 – scientific programme
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BP: Fachverband Biologische Physik
BP 26: Poster Session II
BP 26.38: Poster
Thursday, March 29, 2007, 17:00–19:30, Poster B
Preparation of horizontal black lipid bilayers incorporated in a microfluidics system for microscopy and industrial parallelization — •Tivadar Mach1, Claus Fütterer1, Jürgen Fritz1, Niels Fertig2, Catalin Chimerel1, and Mathias Winterhalter1 — 1International University Bremen, Bremen, Germany — 2Nanion GmbH, München, Germany
A planar black lipid membrane, widely used for electrophysiological studies, is reconstituted on a micron-size glass aperture inside a microfluidic chip, forming a GΩ seal using giant liposome adsorption and rupture. This novel system offers very low noise recordings (under 1 pA RMS at 10 kHz, essentially equal to the open headstage noise in our system), complete control of the measurement environment, access to the bilayer from both sides, the use of μl analyte volumes, and a great potential for automation and parallelization. Minimizing the microfluidics thickness on the lower side of the BLM enables us to approach the bilayer with a 100x objective making concurrent electrophysiological and fluorescence microscopy studies possible.