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Regensburg 2007 – scientific programme

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BP: Fachverband Biologische Physik

BP 28: Biomedical Applications

BP 28.4: Talk

Friday, March 30, 2007, 11:45–12:00, H44

Ellipsometric studies on protein adsorption kinetics — •Christoph Gilow1, Hubert Mantz1, Anthony Quinn1, Karin Jacobs1, Markus Bellion2, and Ludger Santen21Saarland University, Experimental Physics, D-66041 Saarbrücken, Germany — 2Saarland University, Theoretical Physics, D-66041 Saarbrücken, Germany

Adsorption processes of proteins play an important role in many biomedical systems. Examples are the protein films on teeth or tissue compatibility.

From a physical point of view these processes raise a number of challenging questions, e.g., which substrate properties (chemical composition, roughness,…) have an impact on protein adsorption.

We investigated the adsorption kinetics of several salivary proteins in a liquid environment on tailored substrates by means of ellipsometry and found a new type of adsorption kinetics. A comparison to extensive Monte Carlo simulations strongly suggests that long-range contributions to the surface potential lead to conformational changes of the protein on the surface which are responsible for the observed unusual kinetics of the amylase.

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