Regensburg 2007 – scientific programme
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BP: Fachverband Biologische Physik
BP 5: Cell Adhesion
BP 5.6: Talk
Monday, March 26, 2007, 12:15–12:30, H44
Dynamic force spectroscopy on multiple bonds: evaluating rupture force histograms with a master equation model — •Thorsten Erdmann1,3, Sebastien Pierrat2, Pierre Nassoy2, and Ulrich Schwarz1 — 1Center for Modelling and Simulation in the Biosciences (BIOMS),Universität Heidelberg, Im Neuenheimer Feld 293, 69120 Heidelberg, Germany — 2Institut Curie, UMR 168, 26 rue d’Ulm, 75248 Paris Cedex 05, France — 3Fom Institute for Atomic and Molecular Physics (AMOLF), Kruislaan 407, 1098 SJ Amsterdam, The Netherlands
We probe the dynamic strength of multiple parallel biotin-streptavidin adhesion bonds under linear loading using the biomembrane force probe setup for dynamic force spectroscopy. Using multiple rather than single bonds allows a more efficient evaluation of the experimental data. Measured rupture force histograms are compared to results from a master equation model for the stochastic dynamics of bond rupture under load. We extract the average number of bonds ruptured in each experiment as well as characteristic parameters of the adhesion bonds. The analysis shows that the peaks in the measured histograms are not simple multiples of the single bond values, but follow from a convolution procedure which generates different peak positions.