Regensburg 2007 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 9: Regulation and Signaling

BP 9.1: Hauptvortrag

Dienstag, 27. März 2007, 09:30–10:00, H43

Single molecule recognition in regulatory systems — •Robert Ros — Experimental Biophysics, Physics Department, Bielefeld University, 33615 Bielefeld, Germany

Molecular recognition between proteins and nucleic acids is fundamental for many aspects of cellular regulation. For instance, a central issue for the regulation of gene expression is the specific recognition of DNA target sequences by transcription regulators. Furthermore, the protein expression of a cell can be regulated on the post-transcriptional level by the interaction of proteins with RNA. We are using atomic force microscopy (AFM) based force spectroscopy to investigate the interaction of proteins with DNA [1-4] and RNA target sequences at the single molecule level. For analyzing our force spectroscopy data, we apply and improve the concept of chemical bond heterogeneity [5], giving insights into the molecular binding mechanism and resulting in quantitative characterization of the interactions in terms of rate constants and dissociation lengths.

[1] F.W. Bartels et al.; J. Struct. Biol. 143: 145 (2003). [2] B. Baumgarth et al.; Microbiology 151: 259 (2005). [3] R. Eckel et al.; Ang. Chem. Int. Ed.44: 3921 (2005). [4] F.W. Bartels et al.; Biophys. J. (accepted). [5] Raible et al. ; Biophys.J. 90: 3851 (2006).