Regensburg 2007 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 11: INTERNAL SYMPOSIUM Scattering Experiments II

CPP 11.3: Talk

Tuesday, March 27, 2007, 14:45–15:00, H37

Annexin binding to solid supported membranes: a neutron and synchrotron scattering study — Kirstin Seidel, Joachim Rädler, and •Bert Nickel — Dep. für Physik, Ludwig-Maximilians-Universität, München

We have developed a microfluidic setup which allows to prepare and observe solid supported membranes by fluorescence microscopy. Two variants exist, one allows for complementary x-ray reflectivity experiments [1], while the other allows for complementary synchrotron studies at 20 keV. The amount of liquid needed is about 2 ml. As a model system, we have chosen Annexin II, which binds in a Ca dependent way to negatively charged membranes. Here, we report neutron reflectivity experiments at Amor (PSI) and Refsans (FRM-2) and compare them with respective synchrotron studies performed at Hasylab Hamburg and ESRF. The goal of these experiments is to find the configuration of the membrane and the Annexin from the diffraction experiments, while the microscopy experiments allow to determine membrane fluidity with and without Annexin present. Experiments were performed in collaboration with B. Windschiegel and C. Steinem (U. Göttingen). Experiments at Refsans were done in collaboration with GKSS (R. Kampmann et al.)

[1] C. Reich, M. Hochrein, B. Krause, B. Nickel, Review of Scientific Instruments 76, 095103 (2005)