Regensburg 2007 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 22: POSTER: INTERNAL SYMPOSIUM Optical Spectrosopy
CPP 22.6: Poster
Mittwoch, 28. März 2007, 16:00–18:30, Poster B
Incorporation of in vitro Synthesized GPCRs into Peptide Tethered Artificial Membran Sensor Platforms — •Rudolf Robelek1, Marcel Leutenegger2, Theo Lasser2, Wolfgang Knoll1, and Eva-Kathrin Sinner1 — 1Max-Planck-Institute for Polymer Research; Ackermannweg 10; 55128 Mainz; Germany — 2Laboratoire d'optique biomédicale; EPFL; Lausanne; Switzerland
In the last decade the optical spectroscopy techniques and biosensors have made a tremendous progress. Against this background it strikes that the study of complex membrane proteins, including the pharmacologically very important class of G protein coupled receptors, is rarely covered by this development. The main reason therefor is the difficult accessibility and availability of these proteins in biosensoric systems that are adapted for optical spectroscopic techniques. To overcome the named problems we here present a new concept using an in vitro expression strategy to directly insert complex membrane proteins into peptide tethered lipid bilayers. The result of this approach, which is demonstrated for the odorant receptor OR5, is a vectorial insertion of the protein under full conservation of its biological function. The protein / biosensor system can then be characterized in detail by different kinds of optical spectroscopic techniques. In our case we applied SPFS, SEIRAS and TIRF-Imaging to characterize the odorant receptor OR5 in a peptide tBLM. The successful transfer of this concept to further protein examples indicates, that the presented strategy could be a versatile platform to make the broad field of complex membrane proteins available to a multitude of optical spectroscopic techniques.