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BP: Fachverband Biologische Physik

BP 15: Single Molecules

BP 15.7: Vortrag

Mittwoch, 27. Februar 2008, 15:45–16:00, C 243

Fluorescent Nanodiamonds for Biological Applications — •Felix Neugart¹, Andrea Zappe¹, Fedor Jelezko¹, Carsten Tietz¹, Jean Paul Boudou², Anke Krüger³, and Jörg Wrachtrup¹ — ¹3. Physikalisches Institut, Universität Stuttgart, Stuttgart — ²Université Pierre et Marie Curie, Paris VI — ³Christian-Albrechts-Universität, Kiel

Nanodiamonds with nitrogene vacancy (NV) defects as colour centre are a promising candidate for a bright, not toxic, not blinking and not bleaching lable for biological aplications. The NV centre is excited in the green (514nm or 532nm wavelength) and emites around 700nm where living cells show a low autofluorescence. We inserted nandiamonds into living cells via microinjection and endocytosis. The diamonds could be detected by fluorescence as well refraction.

Functionalisation of the nanodiamonds surface is important for labelling as well as for the avoidance of aggregation under physiological conditions. The stability of hydrosols strongly depends on the surface potential of the particles, pH, and solvent salt concentration.

The fluorescence and motional dynamics of single diamond nanocrystals in buffer solution and in living cells is investigated. Stable hydrosols of nanodiamonds in buffer solutions are analysed by fluorescence correlation spectroscopy. [1]

[1] Neugart et al., Nano Letters, (2007)