Berlin 2008 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 26: Posters II
BP 26.13: Poster
Donnerstag, 28. Februar 2008, 17:00–19:30, Poster A
2-Photon laser scanning microscopy of cartilage materials — •Thorsten Bergmann1, Jörg Martini1, Maik Tiemann1, Michael Dickob2, Ronald Schade3, Klaus Liefeith2, Katja Tönsing1, and Dario Anselmetti1 — 1Bielefeld University, Exp. BioPhysics & Appl. NanoSc., Bielefeld, Germany — 2Orthopedic Surgery, Bielefeld, Germany — 3IBA e.V., Department of Biomaterials, Heilbad Heiligenstadt, Germany
2-photon laser scanning microscopy (2PLSM) is a powerful tool for label-free investigation of living cells and strongly scattering tissue samples. In our experiments, strongly scattering native hyaline cartilage has been imaged with this technique using multifocal 2PLSM and analyzed in descanned and non-descanned detection mode. Intensity, wavelength and fluorescence lifetime sensitive detection methods were used for imaging the autofluorescence of the extracellular matrix (ECM) as well as the chondrocytes, the only cells within this tissue. Spectral and lifetime separation of chondrocytes from the ECM allow to quantify the chondrocyte density. The intensity and the structural differences of the detected fluorescence signal from the ECM can be used for differentiation of arthritic and non-arthritic cartilage. Additionally, results of an investigation of collagen scaffolding materials and a comparison concerning chondrocyte density will be discussed.
[1] J. Martini, K. Tönsing, M. Dickob, D. Anselmetti: Proc. of SPIE, 5860: 16-21, 2005 [2] J. Martini, K. Tönsing, M. Dickob, R. Schade, K. Liefeith, D. Anselmetti: Proc. of SPIE, 6089: 274-282, 2006 [3] J. Martini, K. Tönsing, D. Anselmetti: BIOspektrum 5, 489-492, 2006