Berlin 2008 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 26: Posters II

BP 26.17: Poster

Donnerstag, 28. Februar 2008, 17:00–19:30, Poster A

Coherent anti-Stokes Raman scattering (CARS) microscopy - a means to visualize molecular distribution in complex, biological samples — •susana chatzipapadopoulos¹, denis akimov², michael schmitt^{1, 2}, and jürgen popp^{1, 2} — ¹Institute of Photonic Technology, Jena, Germany — ²Institute of Physical Chemistry, Friedrich Schiller University of Jena, Germany

Coherent anti-Stokes Raman scattering (CARS) microscopy is a non linear Raman mapping technique which provides vibrational contrast with high chemical selectivity and high 3D sectioning capability. CARS is applied to complex, biological samples in order to visualize the molecular distribution of various types of molecules with high efficiency, high chemical selectivity and low acquisition times without the need of staining. Unfortunately the CARS signal is not background free and also is derived from the bulk media. In order to develop techniques to yield pure molecular contrast we analyze different, non-linear and linear contrast mechanisms contributing to the CARS image.