Berlin 2008 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 26: Posters II
BP 26.25: Poster
Donnerstag, 28. Februar 2008, 17:00–19:30, Poster A
Dynamic Force Spectroscopy studies of native and synthetic point mutated transcription regulators — •Andre Körnig1, Katrin Wollschläger2, Norbert Sewald2, Dario Anselmetti1, and Robert Ros1 — 1Experimental Biophysics and Applied Nanoscience, Universität Bielefeld, Universitätsstraße 25, 33615 Bielefeld — 2Bioorganic Chemistry, Universität Bielefeld, Universitätsstraße 25, 33615 Bielefeld
For many aspects of cellular regulation the specific interaction between proteins and DNA is fundamental. Especially the recognition of specific DNA target sequences by transcription regulators is a central issue for the regulation of gene expression. We are using atomic force microscope (AFM) based force spectroscopy to investigate the molecular mechanism of the interaction of the transcription regulator PhoB with DNA target sequences on a single molecule level. In order to investigate the contribution of single amino acids to the specificity and strength of the binding as well as the role of the protein environment we compare the wild type protein with point mutants and peptides representing the PhoB recognition helix. This allows a quantitative analysis of the dissociation rates of those complexes and gives insights into the energy landscape of the respective interaction.