Berlin 2008 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 4: Novel Methods
BP 4.2: Vortrag
Montag, 25. Februar 2008, 14:45–15:00, PC 203
SERS Microscopy: Selective and Sensitive Localization of Proteins in Tissue Specimens — •Magdalena Gellner1, Max Schütz1, Carina Jehn1, Florian Baum1, Bernd Küstner1, Carsten Schmuck2, Alexander Marx3, Philipp Ströbel3, and Sebastian Schlücker1 — 1Physikalische Chemie, Julius-Maximilians-Universität, 97074 Würzburg — 2Organische Chemie, Julius-Maximilians-Universität, 97074 Würzburg — 3Pathologisches Institut, Universitätsklinikum, 68167 Mannheim
We have introduced surface–enhanced Raman scattering microscopy (µSERS) as a novel approach to immunohistochemistry. Specifically, the localization of prostate–specific antigen (PSA) in formalin–fixed and paraffin–embedded prostate tissue specimens from patients undergoing prostatectomy for prostate cancer has been demonstrated. In contrast to the use of either dyes or fluorophores as labels, organic molecules as Raman markers on the surface of metal nanoparticles offer unique capabilities for a highly multiplexed detection, because the line width of vibrational transitions is significantly smaller in comparison to electronic transitions. The SERS distance dependence, the SERS selection rules and the specific electronic resonance conditions lead to the fact that only a very few Raman bands from the marker moiety close to the nanoparticle surface are detected. In our case the characteristic Raman signals of the marker were measured in the PSA–(+) epithelial tissue. For negative controls, Raman spectra in the PSA–(-) stroma and lumen were recorded. Further applications for this innovative Raman technique in cell and tumor biology are discussed.