Berlin 2008 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 4: Novel Methods

BP 4.5: Vortrag

Montag, 25. Februar 2008, 15:30–15:45, PC 203

Combination of atomic force microscopy with timecorrelated fluorescence-spectrocopy — •Max Schoengen, Franz-Josef Schmitt, Andrea Matschulat, and Hans Joachim Eichler — Institut für Optik und Atomare Physik, Technische Universität Berlin

The combination of different microscopic techniques delivers completely new possibilities of analysing nanostructures. The topographic analysis performed with Atomic force microscopy can be complemented with time resolved fluorescence spectroscopy and fluorescence microscopy to deliver additional spectroscopic properties of the investigated sample with time and space resolution. With fluorescence microscopy even nanometer scaled structures can be visualised and localized inside the sample. Near field optical techniques like the combination of Foerster Resonance energy transfer (FRET) with AFM can help to investigate simultaneously the topographic and electronic properties of the nanoscaled structures (e.g. membrane proteins). Especially in liquids this kind of AFM combined with FRET technology opens complete new possibilities of analysing (biological) nanostructures like cell membranes.