Berlin 2008 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Posters I
BP 7.38: Poster
Montag, 25. Februar 2008, 17:00–19:30, Poster A
Influence of Receptor Mobility and Micropatterning upon Biomembrane Adhesion — •Susanne Fenz1, Cornelia Monzel1, Sabine Dieluweit1, Kheya Sengupta2, and Rudolf Merkel1 — 1Institute of Bio- and Nanosystems 4: Biomechanics, Research Centre Jülich, Germany — 2CRMC-N (UPR CNRS 7251), Luminy, Marseille, France
The adhesion of cells is a complex process essential for life. It is caused by specific binding between biomolecules that form supramolecular structures at late times. However, the initial steps of cell adhesion, where physical forces dominate, are barely understood. Therefore we developed and quantitatively analyzed a simplified model system. Cell adhesion was mimicked by vesicles, with the specific binding being mediated by the biotin neutravidin complex. Micropatterns of adhesion-competent and repulsive areas were produced by microcontact printing. The adhered vesicle exhibited areas of fluctuating and frozen membrane, corresponding to the underlying pattern. From Reflection Interference Contrast Microscopy (RICM) analysis we obtained the distribution of height fluctuations yielding the potential of interaction. Printed pattern and receptor density were varied systematically. To further investigate the correlation between vesicle adhesion and receptor mobility a supported lipid bilayer was used as substrate. Thus, the lipid coupled receptors were able to diffuse freely. The process of vesicle adhesion was monitored in RICM as well as fluorescence microscopy. We found vesicle adhesion induced distinct protein enrichment in the adhesion disc accompanied by a decrease in diffusivity.