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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 34: POSTERS Polymerphysics

CPP 34.13: Poster

Donnerstag, 28. Februar 2008, 17:00–19:30, Poster A

Influence of the nanoscale lamellar orientation of UHMW-PE surfaces on the protein adsorption — •Thomas Keller, Matthias Grosch, Erik Vater, David Trimbach, and Klaus D. Jandt — Institut für Materialwissenschaft und Werkstofftechnologie (IMT), Friedrich-Schiller-Universtität Jena, Löbdergraben 32, 07743 Jena

The here presented study shows that for thin-melt drawn films of ultra-high molecular weight polyethylene (UHMW-PE) - an implant material already in use - the lamellar crystal arrangement on the surface can be influenced on the nano-scale by processing conditions. These lamellae protrude from the film surface and therefore, such highly oriented UHMW-PE films open up the possibility to investigate the interaction of nano-topographic features with proteins and cells. The in arthroplasty most relevant protein human serum albumin (HSA) was systematically adsorbed on these UHMW-PE surfaces and analyzed by means of atomic force microscopy (AFM). Solutions of different HSA concentrations in PBS buffer were brought onto the highly ordered UHMW-PE surfaces and left for adsorption for different periods of time. By varying the concentration and time for protein adsorption, a partial to full surface coverage was achieved. On the partially HSA-covered UHMW-PE surfaces a preferential alignment of the proteins with the lamellar thickness parallel to the drawing direction was observed. Thus, new insight into the interaction of proteins with nanoscale topographic features of UHMW-PE surfaces was gained.