Parts | Days | Selection | Search | Downloads | Help
CPP: Fachverband Chemische Physik und Polymerphysik
CPP 35: Interfaces
CPP 35.6: Talk
Friday, February 29, 2008, 12:00–12:15, C 130
Activity and Conformation of Lysozyme Adsorbed on Surfaces of Nanodiamond/-silica — •Victor Wei-Keh Wu — Department of Chemical and Materials Engineering, National Kaohsiung University of Applied Sciences, 80782 Kaohsiung, Taiwan — Victor Basic Research Laboratory e. V. Gadderbaumer-Str. 22, 33602 Bielefeld, Germany, Email:victorbres3tw@yahoo.com.tw, Tel:+886-919-300-525.
Coverages and adsorption constants via fluorescence of free lysozyme before and after adsorption on the surfaces of nanodiamond/-silica of diameter 100 nm with their suspension solutions prepared with concentration of 25 mg in 10 mL PPBS of 7 mM at pH=11 have been obtained. 55 and 15 nm2/g BET surfaces of nanodiamond/-silica after adsorptive-dose rate-static-volumetric method were obtained. 12 and 4 nm2 as available surfaces for each lysozyme at saturation threshold 190 and 175 nM for lysozyme and nanodiamond/-silica, respectively, can be derived. Coverages 10∼65 % after adsorption on nanodiamond correspond 10∼75 % of the original lysozyme activity, if effects upon helicity for both at pH=5 and 11 were similar. Lysozyme can possibly keep its helicity well and show its optimal activity, even as surface on nanodiamond is covered nearly 100 % at 700 nM. On the contrary, it on surface of nanosilica can still keep its helicity well, only if its concentration is lower than 100 nM. It might be deformed or changed with its folding structure much in the region of 100-1000 nM. Nanosilica is rather incompetent in the sense of adsorption of lysozyme. Ref. 1. V. W.-K. Wu, CL. 35, 1380 (2006), 2. T.-T. Bao Nguyen, H.-C. Chang, and V. W.-K. Wu, Diamond and Related Materials, 16, 872 (2007).