Berlin 2008 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 9: POSTERS Single Molecules, Biopolymers, Membranes
CPP 9.13: Poster
Montag, 25. Februar 2008, 16:45–19:00, Poster A
Nanopatterning of functional protein arrays — •Mark Schnietz1, Helge Großmann2, Harun Solak3, Robert Tampé2, Andrey Turchanin1, and Armin Gölzhäuser1 — 1Department of Physics, Physics of Supramolecular Systems, University of Bielefeld, D-33615 Bielefeld — 2Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe-University, D-60438 Frankfurt — 3Laboratory for Micro and Nanostructuring, Paul Scherrer Institut, CH-5232 Villigen PSI
The ability to anchor oriented and functional protein arrays at the nanoscale affords useful materials for both fundamental research in biophysics/-chemistry and biotechnological and tissue engineering applications. In this contribution we present a novel approach to the fabrication of protein nanoarrays based on the combination of extreme UV interference lithography (EUV-IL) of self-assembled monolayers for the generation of chemical nanopatterns [1] (top-down) and biochemical tweezers, multivalent surface chelators [2] (tris-nitrilotriacetic acid, tris-NTA), for high affinity capturing of His-tagged proteins (bottom-up). The functionality of the generated protein arrays was demonstrated under physiological conditions via specific, homogeneous, oriented and reversible immobilization of His6-tagged 20S proteasome and fluorescence labeled His10-tagged maltose-binding proteins [3]. Various highly periodic dot and line patterns with areas up to ~10 mm2 and features from 1000 nm to 50 nm were built in this way. [1] A. Turchanin et al., Small 2007. [2] A. Tinazli et al., Chem. Eur. J. 2005, 11, 5249. [3] A. Turchanin et al., Adv. Mater. 2007.